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Image Search Results
Journal: Life Science Alliance
Article Title: Characterisation of the OTU domain deubiquitinase complement of Toxoplasma gondii
doi: 10.26508/lsa.202201710
Figure Lengend Snippet: (A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or Lumitein staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.
Article Snippet: Samples were resolved by SDS–PAGE and visualised with the Silver Stain Plus kit (Bio-Rad) or
Techniques: Construct, Purification, Activity Assay, Modification, Positive Control, SDS Page, Silver Staining, Incubation, Staining