uv stain Search Results


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Gold Biotechnology Inc bright luminescent uv protein gel stain
Bright Luminescent Uv Protein Gel Stain, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biotium liveor dyetm fixable viability staining kits
Liveor Dyetm Fixable Viability Staining Kits, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biotium one step lumitein uv protein gel stain
(A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or <t>Lumitein</t> staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.
One Step Lumitein Uv Protein Gel Stain, supplied by Biotium, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sysmex Corporation cystain uv precise p staining buffer
(A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or <t>Lumitein</t> staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.
Cystain Uv Precise P Staining Buffer, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Partec cystain ultraviolet precise p staining buffer
(A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or <t>Lumitein</t> staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.
Cystain Ultraviolet Precise P Staining Buffer, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Analysis GmbH nuclear extraction buffer quantum stain na uv-5 2-step kit
(A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or <t>Lumitein</t> staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.
Nuclear Extraction Buffer Quantum Stain Na Uv 5 2 Step Kit, supplied by Analysis GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Partec staining buffer partec cystain uv precise p
(A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or <t>Lumitein</t> staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.
Staining Buffer Partec Cystain Uv Precise P, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Partec staining reagent partec cystain uv precise-kit
(A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or <t>Lumitein</t> staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.
Staining Reagent Partec Cystain Uv Precise Kit, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Partec cystain uv precise p high-resolution dna staining kit
(A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or <t>Lumitein</t> staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.
Cystain Uv Precise P High Resolution Dna Staining Kit, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Biolabs Inc oxiselect ™ cellular uv-induced dna damage staining kit
(A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or <t>Lumitein</t> staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.
Oxiselect ™ Cellular Uv Induced Dna Damage Staining Kit, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Partec nuclear staining solution cystain uv-precise kit
(A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or <t>Lumitein</t> staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.
Nuclear Staining Solution Cystain Uv Precise Kit, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PPG Industries c836e35 gunstock uv stain
(A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or <t>Lumitein</t> staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.
C836e35 Gunstock Uv Stain, supplied by PPG Industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or Lumitein staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.

Journal: Life Science Alliance

Article Title: Characterisation of the OTU domain deubiquitinase complement of Toxoplasma gondii

doi: 10.26508/lsa.202201710

Figure Lengend Snippet: (A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or Lumitein staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.

Article Snippet: Samples were resolved by SDS–PAGE and visualised with the Silver Stain Plus kit (Bio-Rad) or One-Step Lumitein UV Protein Gel Stain (Biotium).

Techniques: Construct, Purification, Activity Assay, Modification, Positive Control, SDS Page, Silver Staining, Incubation, Staining